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Venkataraman G Visakh Varma G. Moreover, the occurrence of a carnitine effect is often inconsistent, possibly depending on the experimental diets, particularly the presence of the precursors, age and growth rate of the animal and the general experimental conditions e.
Burtle, ; Litz, ; Schuhmacher et al. In studies with piglets and freshly hatched quail L-carnitine appeared to improve weight gain and feed efficiency only if the metabolic precursors lysine or methionine were deficient in the diets Schuhmacher et al.
However, it seems very unlikely that an amino acidsparing effect on a molar basis was responsible for these findings.
Post-market monitoring The FEEDAP Panel considers that there is no need for a specific post-market monitoring plan, other than the requirements established in the Feed Hygiene Regulation35 and good manufacturing practice.
OJ L 35, 8. Nevertheless, based on residue data obtained from multi-fold doses of the levels typically use, the FEEDAP Panel concluded that typical supplementation of feed with L-carnitine or L-carnitine L-tartrate would not substantially increase human exposure to carnitine from food of animal origin.
L-Carnitine and L-carnitine L-tartrate are not irritant to skin and eyes, nor are they skin sensitisers. LCarnitine and L-carnitine L-tartrate show limited dust formation.
As inhalation toxicity studies are not available, adverse effects in the respiratory tract cannot be fully excluded. L-Carnitine and L-carnitine L-tartrate as a feed additive for all animal species.
October Submitted by Lonza Benelux BV. L-Carnitine 3. L-Carnitine 4. Comments from Member States received through the ScienceNet.
Supplementary information. February Vitamins in Animal Nutrition. ISBN Bremer J, L-Carnitine supplementation of sows during pregnancy improves the suckling behaviour of their offspring.
The influence of two different dosages of L-carnitine on some blood parameters during exercise in trained pigeons. Turnover of carnitine by rat tissues.
Burtle GJ, L-Carnitine enhanced fish growth. Presentation at Carnitine seminar, Lonza, Basel. Effects of dietary L-carnitine supplements on growth and muscle lipid of fingerling channel catfish.
Report to Lonza, Basel. Carnitine intake and excretion in neuromuscular diseases. Radioisotopic determination of L-carnitine content in foods commonly eaten in Western countries.
The effect of L-carnitine on force development of the latissimus dorsi muscle in dogs. Acute effect of L-carnitine on skeletal muscle force tests in dogs.
EFSA Journal 9 6 : , 24pp. European Pharmacopeia PhEur , Levocarnitine, Monograph MG , 7th edition. Falaschini A and Trombetta MF, Supplementation of L-carnitine in pigs: absorption of carnitine and effect on plasma and tissue carnitine concentrations.
The effect of oral L-carnitine supplementation on the muscle and plasma concentrations in the Thoroughbred horse.
Comparative Biochemistry and Physiology. Part A. Effect of training and L-carnitine on long-term exercise in pigeon.
Kurzfassung, A study on the carnitine metabolism of early weaned piglets. Dissertation vet. Bestimmung von L-Carnitin und seiner Ester in Lebensmitteln.
Hamburg, University, Fachbereich Chemie, Dissertation. In vivo studies of intestinal carnitine absorption in rats. Carnitine transport in human intestinal biopsy specimens.
Demonstration of an active transport system. Pharmacokinetics of intravenous and oral bolus doses of L-carnitine in healthy subjects.
Plasma carnitine concentration and uptake into muscle following oral and intravenous administration.
Equine Veterinary Journal, 18, Dietary L-carnitine improves nitrogen utilization in growing pigs fed low energy, fat-containing diets.
A study on the carnitine status of baby quail Coturnix coturnix japonica. Atlantic salmon Salmo salar fed L-carnitine exhibit altered intermediary metabolism and reduced tissue lipid, but no change in growth rate.
Kliemant A, Studies on the carnitine metabolism of Japanese quail Coturnix coturnix japonica. The determination of L-carnitine in several food samples.
Food Chemistry, , Uptake of Lcarnitine by rat jejunal brush border microvillous membrane vesicles. Evidence of passive diffusion.
Lindstedt G and Lindstedt S, On the biosynthesis and degradation of carnitine. Litz H, Examination of the overall effect of a L-carnitine addition to piglet starter diets and to diets for rainbow trout Oncorhynchus mykiss.
Dissertation med. L-Carnitine and carnitine ester transport in the rat small intestine. McDowell LR, Vitamins in Animal and Human Nutrition, 2nd edition.
Metabolism, turnover time, half life, body pool of carnitineC in normal, alloxan diabetic and insulin treated rats. Mitchell ME, Carnitine metabolism in human subjects.
Normal metabolism. Effects of L-carnitine fed during gestation and lactation on sow and litter performance. Effects of L-carnitine fed during lactation on sow and litter performance.
Carnitine in nursery pig diets. Special Publ. Journal of Animal Science, 66 Suppl. Carnitine supplementation for finishing pigs.
Journal of Animal Science, 67 Suppl. The effect of dietary L-carnitine on growth performance and tissue accretion rates in the early weaned pig.
Journal of Animal Science, 72 Suppl. Effect of L-carnitine and soybean oil on growth performance and body composition of early-weaned pigs.
Effect of dietary Lcarnitine on growth performance and body composition in nursery and growing-finishing pigs. Dietary L-carnitine suppresses mitochondrial branched-chain keto acid dehydrogenase activity and enhances protein accretion and carcass characteristics of swine.
Pelletier B, L-Carnitine or vitamin Bt of interest in the dog. Supplementation of sows with L-carnitine during pregnancy and lactation improves growth of the piglets during the suckling period through increased milk production.
Effects of L-carnitine supplementation on milk production, litter gains and back-fat thickness in sows with a low energy and protein intake during lactation.
Kinetic compartmental analysis of carnitine in the human carnitine deficiency syndromes. Evidences for alterations in tissue carnitine transport.
Metabolic fate of dietary carnitine in human adults: identification and quantification of urinary and fecal metabolites.
Rebouche CJ, Effects of increasing dietary L-carnitine on growth performance of weanling pigs. Effects of supplemental dietary carnitine on growth and lipid metabolism of hatchery-reared sea bass Dicentrarchus labrax L.
Carnitine administration to sea bass Dicentrarchus labrax L. Schnitger A, Effect of oral carnitine supplementation on plasma carnitine concentration in the horse.
Carnitin bei Fischen, Ferkeln und Wachteln. Niederkleen, Germany: Wissenschaftlicher Fachverlag Dr. Fleck, pp. Carnitine transport in rat small intestine.
The effect of dietary L-carnitine on the growth performance of the African catfish Clarias gariepinus in relation to dietary lipid.
Fate of tritium-labeled carnitine administered to dogs and rats. American Journal of Physiology, , Authorisation is sought for the use of the two feed additives for all animal species and categories.
Both feed additives are intended to be used in premixtures or added directly into the feedingstuffs and water. None of the Applicants proposed any minimum or maximum concentration of L-Carnitine or LCLT in feedingstuffs or water, as set in the previous regulations.
Even though no performance characteristics are provided, the EURL recommends for official control the European Pharmacopoeia method Ph.
For the determination of L-Carnitine in premixtures, feedingstuffs and water Applicant1 submitted an enzymatic method, specific for the L-Carnitine isomer.
This single-laboratory validated method was further verified by a second independent laboratory. The relative precisions i.
For the determination of L-Carnitine in premixtures, Applicant2 proposed an ion chromatography method with electrical conductivity detection IC-ECD , which does not distinguish between two enantiomers L- and D-Carnitine.
The relative precisions ranging from 4. The relative precisions ranging from 6. For the determination of L-Carnitine in water, Applicant2 proposed a potentiometric titration.
RSDr of 0. Based on the above considerations and the performance characteristics presented, the EURL recommends for official control the following single-laboratory validated and further verified methods 36 The full report is available on the EURL website.
EFSA Journal ;10 5 19 L-Carnitine and L-carnitine L-tartrate for all animal species based on the spectrophotometric method after enzymatic reaction with carnitine-acetyl-transferase enzymatic reaction for the determination of L-Carnitine in premixtures, feedingstuffs and water, together with ion chromatography method with electrical conductivity detection IC-ECD for the determination of L-Carnitine in premixtures; Reversed-Phase High Performance Liquid Chromatography RP-HPLC using a fluorimetric detector for the determination of L-Carnitine in feedingstuffs; potentiometric titration with hydrochloric acid for the determination of L-Carnitine in the water.
The method was single-laboratory validated and further verified. RSDr ranging from 0. RRec ranging from Based on the performance characteristics presented, the EURL recommends for official control the single-laboratory validated and further verified potentiometric back-titration method to determine LCarnitine L-Tartrate LCLT in the feed additive.
Therefore, the EURL considers that all the analytical methods recommended for the determination of L-Carnitine in the matrices investigated are suitable for official control to determine LCLT expressed as L-Carnitine in premixtures, feedingstuffs and water.
Furthermore, it is suggested that L-carnitine improves the oxygen supply and utilisation of the skeletal and heart muscle Liedtke et al.
Accordingly, carnitine might also be important in the case of muscle exercise, particularly under anaerobic conditions Cerretelli and Marconi, L-Carnitine is synthesised endogenously in five enzyme-catalysed steps from its precursor lysine, which provides the carbon backbone, and with methionine as methyl group donor e.
Bremer, , ; Wolf and Berger, ; Tanphaichitr et al. The principles of the synthesis and metabolism of L-carnitine are given in Figure B1.
Based on these observations carnitine is sometimes considered to be conditionally essential. However, up until now it has not been possible to define the requirement for L-carnitine.
After ingestion L-carnitine is absorbed in the small intestine via a sodium-dependent transport system low carnitine concentration or at pharmacological doses of carnitine via passive diffusion as free carnitine and short-chain carnitine esters Shaw et al.
The major portion of all carnitine in the body is found in the skeletal muscle see, for example, Mitchell, Biosynthesis of carnitine in vivo.
Carnitine precursors in the rat. Fatty acid oxidation and cardiac function in the sodium pivalate model of secondary carnitine deficiency.
Cerretelli P and Marconi C, L-Carnitine supplementation in humans. The effects on physical performance.
Lonza Documentation, Lonza, Basel.